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PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (SOLANUM LYCOPERSICUM) UNDER

《农业科学与工程前沿(英文)》 2021年 第8卷 第2期

摘要:

D1 protein turnover and the xanthophyll cycle (XC) are important photoprotective mechanisms in plants that operate under adverse conditions. Here, streptomycin sulfate (SM) and dithiothreitol (DTT) were used in tomato plants as inhibitors of D1 protein turnover and XC to elucidate their photoprotective impacts under sub-high temperature and high light conditions (HH, 35°C, 1000 µmol·m-2·s-1). SM and DTT treatments significantly reduced the net photosynthetic rate, apparent quantum efficiency, maximum photochemical efficiency, and potential activity of photosystem II, leading to photoinhibition and a decline in plant biomass under HH. The increase in reactive oxygen species levels resulted in thylakoid membrane lipid peroxidation. In addition, there were increased non-photochemical quenching and decreased chlorophyll pigments in SM and DTT application, causing an inhibition of D1 protein production at both transcriptional and translational levels. Overall, inhibition of D1 turnover caused greater photoinhibition than XC inhibition. Additionally, the recovery levels of most photosynthesis indicators in DTT-treated plants were higher than in SM-treated plants. These findings support the view that D1 turnover has a more important role than XC in photoprotection in tomato under HH conditions.

 

关键词: D1 turnover / photoinhibition / photoprotection / photosynthesis / tomato / xanthophyll cycle    

PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (

Tao LU, Jiazhi LU, Mingfang QI, Zhouping SUN, Yufeng LIU, Tianlai LI

《农业科学与工程前沿(英文)》   页码 262-279 doi: 10.15302/J-FASE-2021383

摘要: D1 protein turnover and the xanthophyll cycle (XC) are important photoprotective mechanisms in plants that operate under adverse conditions. Here, streptomycin sulfate (SM) and dithiothreitol (DTT) were used in tomato plants as inhibitors of D1 protein turnover and XC to elucidate their photoprotective impacts under sub-high temperature and high light conditions (HH, 35°C, 1000 µmol·m ·s ). SM and DTT treatments significantly reduced the net photosynthetic rate, apparent quantum efficiency, maximum photochemical efficiency, and potential activity of photosystem II, leading to photoinhibition and a decline in plant biomass under HH. The increase in reactive oxygen species levels resulted in thylakoid membrane lipid peroxidation. In addition, there were increased non-photochemical quenching and decreased chlorophyll pigments in SM and DTT application, causing an inhibition of D1 protein production at both transcriptional and translational levels. Overall, inhibition of D1 turnover caused greater photoinhibition than XC inhibition. Additionally, the recovery levels of most photosynthesis indicators in DTT-treated plants were higher than in SM-treated plants. These findings support the view that D1 turnover has a more important role than XC in photoprotection in tomato under HH conditions.

关键词: D1 turnover     photoinhibition     photoprotection     photosynthesis     tomato     xanthophyll cycle    

Analysis of a 1 kW organic Rankine cycle using a scroll expander for engine coolant and exhaust heat

Yiji LU, Anthony Paul ROSKILLY, Long JIANG, Longfei CHEN, Xiaoli YU

《能源前沿(英文)》 2017年 第11卷 第4期   页码 527-534 doi: 10.1007/s11708-017-0516-0

摘要: The development of engine waste heat recovery technologies attracts ever increasing interests due to the rising strict policy requirements and environmental concerns. This paper presented the study of engine coolant and exhaust heat recovery using organic Rankine cycle (ORC). Eight working fluids were selected to evaluate and compare the performance of the integrated waste heat recovery system. Rather than the conventional engine ORC system mainly focusing on the utilization of exhaust energy, this work proposed to fully use the engine coolant energy by changing the designed parameters of the ORC system. The case study selected a small engine as the heat source to drive the ORC system using a scroll expander for power production. The evaluation results suggest that under the engine rated condition, the solution to fully recover the engine coolant energy can achieve a higher power generation performance than that of the conventional engine ORC system. The results suggest that adding a recuperator to the ORC system can potentially improve the system performance when the working fluids are dry and the overall dumped heat demand of the system can be reduced by 12% under optimal conditions. When the ORC evaporating and condensing temperature are respectively set at 85°C and 30°C, the integrated engine waste heat recovery system can improve the overall system efficiency by 9.3% with R600, R600a or -Pentane as the working fluid.

关键词: organic Rankine cycle     scroll expander     coolant and exhaust recovery     internal combustion engine    

基于RGB-D传感器的室内三维建模及应用研究综述 Review Article

原志路1,李游1,汤圣君1,李明2,郭仁忠1,王伟玺1

《信息与电子工程前沿(英文)》 2021年 第22卷 第6期   页码 815-826 doi: 10.1631/FITEE.2000097

摘要: 随着消费级RGB-D摄像机的快速发展,真实世界的室内三维场景建模和机器人应用越来越受到重视。然而,室内三维场景建模仍具有挑战性,因室内物体结构可能具有较高复杂性,在此情况下,消费者级传感器采集的RGB-D数据质量需进一步提升。近年来,在提高消费者级传感器采集的RGB-D数据质量方面,有很多值得关注的研究。本文介绍了室内场景建模方法的最新进展、室内公共数据集和库以及RGB-D设备的典型应用,包括室内定位和紧急疏散。

关键词: 三维室内制图;RGB-D;室内定位;施工监测;应急疏散    

CHARACTERISTICS OF HERBIVORY/WOUND-ELICITED ELECTRICAL SIGNAL TRANSDUCTION IN TOMATO

《农业科学与工程前沿(英文)》 2021年 第8卷 第2期

摘要:

Electrical signals commonly occur in plants in response to various environmental changes and have a dominant function in plant acclimation. The transduction of wound-elicited electrical signals in the model plant species Arabidopsis has been characterized but the characteristics of electrical signal transduction in response to herbivory or wounding in crop species remain unknown. Here, the features of electrical signals elicited by insect herbivory and wounding in tomato were investigated. Unlike those in Arabidopsis, wounding tomato leaves did not cause leaf-to-leaf electrical signal transduction. In contrast, electrical signals elicited in response to petiole wounding were stronger and more strongly transduced. Leaflet wounding also activated electrical signal transduction and jasmonic acid (JA) signaling within the whole compound leaf. It was also demonstrated that tomato glutamate receptor-like 3.3 (GLR3.3) and GLR3.5 mediated leaflet-to-leaflet electrical signal transduction. Herbivory-induced JA accumulation and Helicoverpa armigera resistance were reduced in glr3.3/3.5 plants. This work reveals the nature of electrical signal transduction in tomato and emphasizes the key roles of GLR3.3 and GLR3.5 in electrical signal transduction and JA signaling activation.

 

关键词: electrical signal / glutamate receptor-like / herbivory / jasmonic acid / tomato    

The role of CDK1 siRNA interference in cell cycle and cell apoptosis

Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,

《医学前沿(英文)》 2009年 第3卷 第4期   页码 384-389 doi: 10.1007/s11684-009-0070-1

摘要: In the present report, cyclin-dependent kinase1 (CDK1) siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis. The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000. The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction (PCR) and Western blot, respectively. The cell cycle was analyzed by using DNA content analysis by flow cytometry. Cell apoptosis was detected by the Annexin V/PI method. The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain. CDK1 gene was successfully silenced by its siRNA, and the CDK1 protein expression level was decreased significantly, especially from 48thh to 60thh after transfection. The DNA content analysis showed that transfection of CDK1 siRNA led to cells accumulating in G/M phase. There was no significant difference in the apoptotic rate between transfected cells and the control cells after transfection of CDK1 siRNA for 48 or 60h. More double nucleus or multinucleus cells could be seen under the microscopy among the transfected cells. The decreased CDK1 expression by siRNA silencing gave rise to cell cycle arrest in G/M phase but did not induce apoptosis.

关键词: cyclin-dependent kinase1     siRNA interference     cell cycle     apoptosis    

nano zinc oxide for active and intelligent packaging: comparison of anthocyanins source from purple tomato

《化学科学与工程前沿(英文)》 2023年 第17卷 第6期   页码 704-715 doi: 10.1007/s11705-022-2270-z

摘要: The multifunctional films was prepared by blending chitosan and nano-ZnO with purple tomato anthocyanins or black wolfberry anthocyanins. The properties of films functioned by anthocyanins source and nano-ZnO content were studied. It was found purple tomato anthocyanins showed more significant color change against pH than black wolfberry anthocyanins. The nano-ZnO were widely dispersed in matrix and enhanced the compatibility of anthocyanins with chitosan. However, the anthocyanins source influenced the properties of the films more slightly than nano-ZnO addition. The tensile strength, antioxidant and antibacterial effects of the chitosan films dramatically increased after cooperated by nano-ZnO and anthocyanins, which also enhanced with increase of nano-ZnO content, whereas the elongation at break of the composite films decreased. Especially, the anthocyanin and nano-ZnO promoted the antibacterial activity of films synergistically. Composite films made from black wolfberry anthocyanins exhibited higher mechanical performance than those made from purple tomato anthocyanins but weaker antibacterial effects. The purple tomato anthocyanins/chitosan and nano-ZnO/purple tomato anthocyanins/chitosan films effectively reflected pork spoilage, changing their colors from dark green to brown, indicating the potential for applications in active and intelligent food packaging.

关键词: bio-based     multifunction     colorimetric indicator     active and intelligent packaging    

Wastewater treatment meets artificial photosynthesis: Solar to green fuel production, water remediation

《环境科学与工程前沿(英文)》 2022年 第16卷 第4期 doi: 10.1007/s11783-022-1536-5

摘要:

• Mitigating energy utilization and carbon emission is urgent for wastewater treatment.

关键词: Wastewater treatment     Artificial photosynthesis     Microbial photoelectrochemical (MPEC) system     Carbon neutral     Renewable energy    

FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

《农业科学与工程前沿(英文)》 2021年 第8卷 第2期

摘要:

Fruit cracking is a major disorder that affects the integrity of fruit and reduces the commercial value of tomato and other fleshy fruit. Here, we have found a novel fruit ‘netted-cracking’ (FNC) phenotype in tomato introgression line IL4-4 which is present in neither the donor parent (LA0716) nor the receptor parent (M82). An F2 population was generated by crossing IL4-4 with M82 to genetically characterize the FNC gene and this showed that a single dominant gene determined fruit netted-cracking. Further map-based cloning narrowed down the FNC locus to a 230 kb region on chromosome 4. Sequencing and annotation analysis show that FNC(Solyc04 g082540) was the most likely candidate gene. Functional characterization of FNC by overexpressing FNCAC and FNCIL4-4resulted in the fruit netted-cracking phenotype, suggesting that the FNC transcript level results in the functional gain of fruit netted-cracking. These findings were further confirmed by FNC ortholog in netted-cracking pepper and melon, indicating a common regulatory mechanism in different plant species. Furthermore, cytoplasm and nucleus-localized FNC indicates increased expression of genes involved in suberin, lignin, lipid transport and cell wall metabolism. These findings provide novel genetic insights into fruit netted-cracking and offer a way to promote molecular improvement toward cracking resistant cultivars.

 

关键词: fine mapping / fruit netted-cracking / introgression line / transcript level    

基于三维参数模型重建的心脏病理视觉识别 Research Article

肖金肖1,李岩松1,田沄1,徐冬溶2,3,李鹏辉1,赵世凤1,潘云鹤3

《信息与电子工程前沿(英文)》 2022年 第23卷 第9期   页码 1324-1337 doi: 10.1631/FITEE.2200102

摘要: 心脏图像的视觉识别对于心脏病理诊断和治疗具有重要意义。由于可用标注数据集有限,传统方法通常基于三维心脏图像的二维切片对病理分类特征进行提取,难以确保心脏解剖结构的整体一致性。为此,本文提出一种基于三维参数模型重建的心脏病理分类方法。首先,基于收缩末期和舒张末期时相心脏图像的多个三维心脏成像数据重建三维心脏模型。其次,基于重建的三维心脏模型,通过统计形状模型方法构建三维参数模型。然后,基于三维统计形状模型及其视觉知识约束对心脏数据进行增强。最后,提取不同时相的三维心脏模型的形状和运动特征,对心脏病理进行分类。在STACOM公开挑战赛的ACDC数据集上的实验验证了所提方法的优越性和有效性。

关键词: 三维视觉知识;三维参数模型;心脏病理诊断;数据增强    

Magnetic Co-doped 1D/2D structured -FeO/MoS effectively activated peroxymonosulfate for efficient abatement

《环境科学与工程前沿(英文)》 2023年 第18卷 第3期 doi: 10.1007/s11783-024-1797-2

摘要:

● Magnetic Co- γ -Fe2O3/MoS2 were prepared via facile hydrothermal methods.

关键词: Magnetic Co-γ-Fe2O3/MoS2     Hydrothermal method     Bisphenol A     Degradation pathways     Toxicity analysis    

VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

《化学科学与工程前沿(英文)》 2016年 第10卷 第4期   页码 542-551 doi: 10.1007/s11705-016-1599-6

摘要: We present the microbial green synthesis of silver nanoparticles (NPs) by VITHM1 strain (MTCC No. 12465). The secondary metabolites in the cell free supernatant of this bacterium when incubated with 1 mmol/L AgNO , mediated the biological synthesis of AgNPs. The synthesized AgNPs were characterized by UV-visible spectrum, X-ray diffraction (XRD), atomic force microscope, scanning electron microscopy equipped with energy dispersive spectroscopy, transmission electron microscopy, FT-IR spectroscopy, dynamic light scattering and zeta potential. They were highly stable and, spherical in shape with the average size of 30?50 nm. The secondary metabolites involved in the formation of AgNPs were identified gas chromatography-mass spectrography. The 3D structure of the unit cell of the synthesized AgNPs was determined using XRD data base. The synthesized AgNPs exhibited significant antibacterial activity against tested bacterial pathogens, and did not show haemolysis on human red blood cells. This green synthesis could provide a new platform to explore and use AgNPs as antibacterial therapeutic agents.

关键词: Streptomyces ghanaensis VITHM1     nanoparticles     3D structure     antibacterial activity    

蓄滞洪区洪水演进一、二维数值仿真及其在洼淀联合调度中的应用

李大鸣,林毅,周志华

《中国工程科学》 2010年 第12卷 第3期   页码 82-89

摘要:

为了实现蓄滞洪区内各洼淀实时洪水调度,考虑到蓄滞洪区内复杂河网与洼淀交错的情况,建立了河道、滞洪区联合计算的一、二维衔接洪水演进数学模型,在考虑铁路、公路、堤防及桥涵等阻水建筑物等复杂边界条件下,采用有限体积法进行离散和求解。结合蓄滞洪区实时洪水调度仿真系统的总体框架,建立了模拟洪水在蓄滞洪区内任意位置的水位、流速、淹没水深、历时等演进过程的一、二维洪水演进数值仿真系统。结合大清河实测资料对东淀滞洪区洪水演进进行了模型验证,结果基本吻合,并应用该系统对大清河滞洪区五洼联合调度方案进行了实时模拟,分析了洼淀联合调度下出现的倒淀现象,对不同方案进行了比较分析,实现了实时的洪水调度分析。

关键词: 蓄滞洪区     洪水演进     一、二维数值仿真     有限体积法     联合调度    

Photosynthesis and related metabolic mechanism of promoted rice (Oryza sativa

Yingdan Zhang, Na Liu, Wei Wang, Jianteng Sun, Lizhong Zhu

《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1282-5

摘要: Abstract • The rice growth was promoted by nano-TiO2 of 0.1–100 mg/L. • Nano-TiO2 enhanced the energy storage in photosynthesis. • Nano-TiO2 reduced energy consumption in carbohydrate metabolism and TCA cycle. Titanium dioxide nanoparticle (nano-TiO2), as an excellent UV absorbent and photo-catalyst, has been widely applied in modern industry, thus inevitably discharged into environment. We proposed that nano-TiO2 in soil can promote crop yield through photosynthetic and metabolic disturbance, therefore, we investigated the effects of nano-TiO2 exposure on related physiologic-biochemical properties of rice (Oryza sativa L.). Results showed that rice biomass was increased >30% at every applied dosage (0.1–100 mg/L) of nano-TiO2. The actual photosynthetic rate (Y(II)) significantly increased by 10.0% and 17.2% in the treatments of 10 and 100 mg/L respectively, indicating an increased energy production from photosynthesis. Besides, non-photochemical quenching (Y(NPQ)) significantly decreased by 19.8%–26.0% of the control in all treatments respectively, representing a decline in heat dissipation. Detailed metabolism fingerprinting further revealed that a fortified transformation of monosaccharides (D-fructose, D-galactose, and D-talose) to disaccharides (D-cellobiose, and D-lactose) was accompanied with a weakened citric acid cycle, confirming the decrease of energy consumption in metabolism. All these results elucidated that nano-TiO2 promoted rice growth through the upregulation of energy storage in photosynthesis and the downregulation of energy consumption in metabolism. This study provides a mechanistic understanding of the stress-response hormesis of rice after exposure to nano-TiO2, and provides worthy information on the potential application and risk of nanomaterials in agricultural production.

关键词: Nano-TiO2     Rice     Photosynthesis     Metabolomics     Energy storage    

Visible light responsive photocatalysts developed by substitution with metal cations aiming at artificial photosynthesis

《能源前沿(英文)》 2021年 第15卷 第3期   页码 568-576 doi: 10.1007/s11708-021-0774-8

摘要: To solve resource, energy, and environmental issues, development of sustainable clean energy system is strongly required. In recent years, hydrogen has been paid much attention to as a clean energy. Solar hydrogen production by water splitting using a photocatalyst as artificial photosynthesis is a promising method to solve these issues. Efficient utilization of visible light comprised of solar light is essential for practical use. Three strategies, i.e., doping, control of valence band, and formation of solid solution are often utilized as the useful methods to develop visible light responsive photocatalysts. This mini-review introduces the recent work on visible-light-driven photocatalysts developed by substitution with metal cations of those strategies.

关键词: visible light responsive photocatalyst     water splitting     artificial photosynthesis: metal ion substitution    

标题 作者 时间 类型 操作

PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (SOLANUM LYCOPERSICUM) UNDER

期刊论文

PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (

Tao LU, Jiazhi LU, Mingfang QI, Zhouping SUN, Yufeng LIU, Tianlai LI

期刊论文

Analysis of a 1 kW organic Rankine cycle using a scroll expander for engine coolant and exhaust heat

Yiji LU, Anthony Paul ROSKILLY, Long JIANG, Longfei CHEN, Xiaoli YU

期刊论文

基于RGB-D传感器的室内三维建模及应用研究综述

原志路1,李游1,汤圣君1,李明2,郭仁忠1,王伟玺1

期刊论文

CHARACTERISTICS OF HERBIVORY/WOUND-ELICITED ELECTRICAL SIGNAL TRANSDUCTION IN TOMATO

期刊论文

The role of CDK1 siRNA interference in cell cycle and cell apoptosis

Hui XIAO PhD, Ming TIAN MM, Junna GE MM, Xin Wei MD, Zhaoming LI MM, Xiaolan LI MS, Deding TAO PhD, Junbo HU MD, Jianping GONG MD,

期刊论文

nano zinc oxide for active and intelligent packaging: comparison of anthocyanins source from purple tomato

期刊论文

Wastewater treatment meets artificial photosynthesis: Solar to green fuel production, water remediation

期刊论文

FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

期刊论文

基于三维参数模型重建的心脏病理视觉识别

肖金肖1,李岩松1,田沄1,徐冬溶2,3,李鹏辉1,赵世凤1,潘云鹤3

期刊论文

Magnetic Co-doped 1D/2D structured -FeO/MoS effectively activated peroxymonosulfate for efficient abatement

期刊论文

VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

期刊论文

蓄滞洪区洪水演进一、二维数值仿真及其在洼淀联合调度中的应用

李大鸣,林毅,周志华

期刊论文

Photosynthesis and related metabolic mechanism of promoted rice (Oryza sativa

Yingdan Zhang, Na Liu, Wei Wang, Jianteng Sun, Lizhong Zhu

期刊论文

Visible light responsive photocatalysts developed by substitution with metal cations aiming at artificial photosynthesis

期刊论文